Research and Implementation of Real Time Fluorescent Nucleic Acid Amplification Detection Technology
نویسندگان
چکیده
منابع مشابه
Microfluidic devices for nucleic acid (NA) isolation, isothermal NA amplification, and real-time detection.
Molecular (nucleic acid)-based diagnostics tests have many advantages over immunoassays, particularly with regard to sensitivity and specificity. Most on-site diagnostic tests, however, are immunoassay-based because conventional nucleic acid-based tests (NATs) require extensive sample processing, trained operators, and specialized equipment. To make NATs more convenient, especially for point-of...
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Real-time isothermal nucleic acid sequence-based amplification (RT-NASBA) was applied to the detection of Mycoplasma pneumoniae. In vitro-generated M. pneumoniae RNA was used to assess the sensitivity of the assay. The 95% hit rate was 148 molecules of M. pneumoniae RNA in the amplification and 10(4) molecules of in vitro-generated RNA after nucleic acid extraction. The sensitivity of the RT-NA...
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ژورنال
عنوان ژورنال: Journal of Applied Virology
سال: 2014
ISSN: 2306-6210,2305-5154
DOI: 10.21092/jav.v3i2.44